DISSECTION OF AFFINITY CAPTURED LINE-1 MACROMOLECULAR COMPLEXES

Long Security Interspersed Nuclear Element-1 (LINE-1, L1) is a mobile genetic element active in human genomes.L1-encoded ORF1 and ORF2 proteins bind L1 RNAs, forming ribonucleoproteins (RNPs).These RNPs interact with diverse host proteins, some repressive and others required for the L1 lifecycle.Using differential affinity purifications, quantitati